Influence of lipopolysaccharide on the biological characteristics of skin fibroblasts and its potential role in wound healing

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 20; no. 9; p. 873
• Main Authors: Yang, Hongming, Li, Fengyu, Chai, Jiake
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2006
• Subjects: Adolescent; Adult; Cell Proliferation; Cells, Cultured; Child; Child, Preschool; Collagen - biosynthesis; Female; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Humans; Lipopolysaccharides - pharmacology; Male; Middle Aged; Skin - cytology; Wound Healing - drug effects
• ISSN: 1002-1892

To investigate the influence of lipopolysaccharide (LPS) on the proliferation and collagen synthesis of normal human skin fibroblasts so as to elucidate its relation with skin wound healing. Fibroblasts were isolated and cultured in vitro, and then exposed to different doses of LPS(0.005, 0.010, 0.050, 0.100, 0.500, and 1.000 microg/ml) from E. coli055:B5 respectively. Then the absorbance (A) value of fibroblasts was determined with the colorirneteric thiazolyl blue (MTT) assay, and the cell number was counted under inverted phase contrast microscope from the 1st day to the 9th day after LPS administration, and collagen synthesis of fibroblasts in culture medium was measured with the method of pepsin digestion after incorporation of 3H-proline into stable, single-layered, confluent fibroblasts at 7 days after LPS administration. Compared with control group, A value increased with the increasing concentration of LPS (0.005 microg/ml-0.500 microg/ml) and LPS of 0.100 microg/ml group had the strongest effect. Th