Dynamic study on effect of Chinese medicine compound Yiliuyin on angiogenesis in transplanted S180 tumor of mouse

• Published in: Zhongguo zhongyao zazhi Vol. 34; no. 2; p. 212
• Main Authors: Han, Zhipeng, Wang, Runtian, Li, Tiemin, Yang, Zhiqiang, Cui, Cheng, Ding, Junying, Zhang, Zhengzheng, Deng, Yuqing, Wang, Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2009
• Subjects: Administration, Oral; Animals; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Endostatins - metabolism; Female; Gene Expression Regulation, Neoplastic - drug effects; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Neoplasms - blood supply; Neoplasms - drug therapy; Neoplasms - genetics; Neoplasms - pathology; Neovascularization, Pathologic - drug therapy; Neovascularization, Pathologic - pathology; Vascular Endothelial Growth Factor A - metabolism; Vascular Endothelial Growth Factor Receptor-2 - metabolism
• ISSN: 1001-5302

To investigate the dynamic changes in angiogenesis within the tumor tissue of mice bearing S180 tumor at different day-points of oral administration with a Chinese medicine compound "Yiliuyin" (YLY) and to explore the anti-tumor mechanisms of YLY in vivo. Fifty-six BALB/c mice were divided into YLY group and control group (28 mice/group) and each group was divided into four subgroups (7 mice/subgroup), randomly. After 24 hrs of inoculation with S180 tumor cells subcutaneously in the right axilla, YLY in the mice of YLY group and equal volume of cold boiled-water in the mice of control group were administered orally twice every day, 0.5 mL each time. The mice of one subgroup from the two groups apiece were killed at 10, 20, 30 th and 40 th day-point of oral administration, respectively. The tumors were isolated and were made into paraffin embedded sections. The dynamic changes of the angiogenesis (CD34 staining), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGFR-2)