Induction of anti-leukemic immunity of dendritic cells derived from multidrug resistant leukemia K562/A02 cells with high expression of P-glycoprotein and sensitive K562 cells

This study was aimed to investigate and compare the anti-leukemic effect mediated by dendritic cells (DC) derived from multidrug resistant (MDR) leukemia K562/A02 cells with high expression of p-glycoprotein (P-gp) and sensitive K562 cells. Multidrug resistant K562/A02 cell line and sensitive K562 c...

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Bibliographic Details
Published inZhongguo shi yan xue ye xue za zhi Vol. 13; no. 6; p. 1018
Main Authors Zhang, Yan-Ming, Zhang, Lian-Sheng, Zhang, Yu-Fang, Yi, Liang-Cai, Chai, Ye, Song, Fei-Xue, Zeng, Peng-Yun, Liu, Ying
Format Journal Article
LanguageChinese
Published China 01.12.2005
Subjects
Antigens, CD - analysis
Antigens, CD1 - analysis
ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis
B7-1 Antigen - analysis
B7-2 Antigen - analysis
CD83 Antigen
Cell Differentiation - drug effects
Cell Differentiation - immunology
Cytotoxicity, Immunologic
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - metabolism
Doxorubicin - pharmacology
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Flow Cytometry
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Humans
Immunoglobulins - analysis
Interleukin-4 - pharmacology
K562 Cells
Leukemia - immunology
Leukemia - pathology
Membrane Glycoproteins - analysis
Tumor Necrosis Factor-alpha - pharmacology
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Summary:This study was aimed to investigate and compare the anti-leukemic effect mediated by dendritic cells (DC) derived from multidrug resistant (MDR) leukemia K562/A02 cells with high expression of p-glycoprotein (P-gp) and sensitive K562 cells. Multidrug resistant K562/A02 cell line and sensitive K562 cell line from chronic myeloid leukemia (CML) were induced for differentiating to DC in complete RPMI 1640 culture medium supplemented with GM-CSF (1 000 U/ml), IL-4 (500 U/ml) and TNF-alpha (100 ng/ml) for 14 days. The morphologic features of DC were observed by means of optical microscopy and the phenotype of DC was detected by flow cytometry. T-cell stimulating activity was determined by allogeneic lymphocyte reaction (allo-MLR). Cytotoxic activity was measured by MTT assay. The results indicated that DC derived from K562/A02 cells and K562 cells similarly showed the typical morphology of dendritic cell and expressed the surface differentiation antigens and costimulatory molecules CD1a, CD83, HLA-DR, CD80 and CD8
ISSN:1009-2137