The characterization of Th1/Th2 profile in end-stage renal disease patients and the correlation with the apoptosis of T lymphocyte

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 22; no. 6; p. 763
• Main Authors: Xie, De-Qiong, Gan, Hua, Du, Xiao-Gang, Li, Zheng-Rong, Wu, Jiang
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2006
• Subjects: Annexin A5 - metabolism; Apoptosis; beta 2-Microglobulin - blood; Case-Control Studies; Female; Gene Expression Regulation; Humans; Interferon-gamma - metabolism; Interleukin-4 - metabolism; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - immunology; Kidney Failure, Chronic - metabolism; Kidney Failure, Chronic - pathology; Leukocytes, Mononuclear - metabolism; Male; Microscopy, Electron, Transmission; Middle Aged; Th1 Cells - immunology; Th1 Cells - metabolism; Th1 Cells - pathology; Th1 Cells - ultrastructure; Th2 Cells - immunology; Th2 Cells - metabolism; Th2 Cells - pathology; Th2 Cells - ultrastructure
• ISSN: 1007-8738

To investigate the characterization of Th1/Th2 profile in patients with end-stage renal disease (ESRD) and the correlation with the apoptosis of the peripheral blood T cells; and to study the influence of different dialysis membranes on the apoptosis of T lymphocyte. T cells from 10 non-dialyzed (ND) patients, 45 maintenance hemodialysis patients with cellulose acetate (CA) membrane, low-flux (PS-LF) and high-flux polusulfone (PS-HF) membrane, and 8 healthy volunteers (HC) were separated and stimulated with PHA for 24 hours in vitro. Then the apoptosis of T cells and supernatants levels of IFN-gamma and IL-4 were detected by Flow cytometry (FCM) and ELISA. In ESRD patients, the expression of Annexin V in T lymphocyte was higher than that of group HC (P<0.05), group CA was higher than group PS-HF and PS-LF (P<0.05). The level of IFN-gamma of ESRD patients was decreased significant compared with that in group HC (P<0.05), and there was negative correlation. between the Annexin V and IFN-gamma. IL-4 was increase