Detection of 16S ribosomal RNA gene of bacteria in prostate tissues of adults

To investigate the role of bacteria in chronic prostatitis. Complete specimens of prostate were obtained from 140 organ donors, aged 20 - 35, at autopsy. A piece of tissue was collected from the peripheral zone of prostate from each specimen and was divided into 2 parts to undergo pathological exami...

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Bibliographic Details
Published inZhong hua yi xue za zhi Vol. 86; no. 14; p. 976
Main Authors Xie, Hui, Huang, Hui-cong, Yang, Yi-rong, Wu, Jian-bo, He, Qiu-xiang, Zhu, Qi-jian, Chen, Jian-ou, Wang, Si-qi, Li, Cheng-di, Xia, Peng, Shen, Long-jie
Format Journal Article
LanguageChinese
Published China 11.04.2006
Subjects
Adult
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Humans
Male
Polymerase Chain Reaction
Prostate - microbiology
Prostatitis - microbiology
RNA, Ribosomal, 16S - genetics
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Summary:To investigate the role of bacteria in chronic prostatitis. Complete specimens of prostate were obtained from 140 organ donors, aged 20 - 35, at autopsy. A piece of tissue was collected from the peripheral zone of prostate from each specimen and was divided into 2 parts to undergo pathological examination and PCR so as to detect the 16S ribosomal RNA (16S rRNA) gene of bacteria. Focal mild inflammation was shown in 46 of the 104 specimens (32.9%), including interstitial inflammation in 42 specimens, inflammation in both interstitial and body of gland in 3 specimens, and perigladulitis in 1 specimen. Twenty-seven of the 140 specimens (19.3%) were positive in 16S rRNA gene. The positive rate of 16S rRNA gene of the specimens with prostatitis was 48.9%, significantly higher than that of the specimens without prostatitis (5.3%, P < 0.001). Bacteria may play an important role in the pathogenesis of chronic prostatitis.
ISSN:0376-2491