The protective effects of ginsenoside RG1 and Rb1 against damage of HaCaT cells by ultraviolet B

• Published in: Yao hsüeh hsüeh pao Vol. 41; no. 9; p. 905
• Main Authors: Cheng, Jun-lin, Zhou, Li-ming, Guan, Xiao-lin, Kuang, Xiang-hong, Zhu, Ling
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2006
• Subjects: Apoptosis - drug effects; Apoptosis - radiation effects; Cell Line; Cell Survival - drug effects; Cell Survival - radiation effects; DNA Damage - drug effects; DNA Damage - radiation effects; Dose-Response Relationship, Radiation; Ginsenosides - isolation & purification; Ginsenosides - pharmacology; Humans; Keratinocytes - cytology; Keratinocytes - drug effects; Keratinocytes - radiation effects; Panax - chemistry; Plants, Medicinal - chemistry; Protective Agents - isolation & purification; Protective Agents - pharmacology; Ultraviolet Rays - adverse effects
• ISSN: 0513-4870

To investigate the survival rate and the level of HaCaT cells damage with ultraviolet B (UVB) radiation at various doses, and observe the protective effects of ginsenoside Rg1 and Rb1 in vitro. MTT assay was employed to analyze the cell survival rate after UVB radiation of 30, 60, 90 and 120 mJ x cm(-2). The damage of nucleolus and the protective effects of ginsenoside Rg1 and Rb1 were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay (SCGE). It was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ x cm(-2) to 120 mJ x cm(-2). At the dose of 20 microg x mL(1-), obvious protective effect of ginsenoside Rg1 and Rb1 can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage. UVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rg1 and Rb1 can relief the damage.