Distribution of hepatitis B virus genotype among population of Dong, Miao minority and Han in Guizhou

• Published in: Zhonghua shi yan he lin chuang bing du xue za ji Vol. 18; no. 3; p. 230
• Main Authors: Ding, Jing-juan, Peng, Liang, Zhang, Quan, Li, Zhong, Tang, Guang-peng
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2004
• Subjects: Adolescent; Adult; China - ethnology; Female; Genotype; Hepatitis B - ethnology; Hepatitis B - virology; Hepatitis B virus - classification; Hepatitis B virus - genetics; Hepatitis B virus - isolation & purification; Humans; Male; Middle Aged; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Species Specificity; China
• ISSN: 1003-9279

To study the distribution of hepatitis B virus (HBV) genotype in population of Dong, Miao minority and Han in Guizhou. S region nucleotides were compared in 127 strains whole sequence of HBV and three restriction enzymes which can be used for genotyping were found by DNA software analysis system. The partial gene fragment of HBV S region was amplified by nested polymerase chain reaction (nPCR). The products were digested with Mbo I, BstN I or BsmA I and subjected to electrophoresis on agarose gel, respectively. The patterns of restriction fragment length polymorphism (RFLP) were analysed. The genotypes were determined by nPCR-RFLP in 166 asymptomatic HBV carriers (ASC), including 48 Dong minority, 52 Miao minority and 66 Han subjects. Some of the ASC were also analysed by direct sequencing of PCR products. The nPCR-RFLP method was simple and accurate. Of the 166 ASC, 138 (83.13%) were genotype B, and 28 (16.87%) were genotype C. Of the 48 Dong minority subjects, 47 (97.2%) were genotype B and 1 (2.08%) was ty