Identification of oligopeptides mimicking the virus attachment protein of hantaan virus

To identify and characterize the epitope associated with the virus attachment protein (VAP) of hantaan virus. The monoclonal antibody 3G1 was used as the ligand to biospan from a phage-displayed 12-amino acid peptide library, then the positive phage clones were chosen and sequenced. The amino acid s...

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Bibliographic Details
Published inZhonghua shi yan he lin chuang bing du xue za ji Vol. 19; no. 1; p. 58
Main Authors Lü, Xin, Xue, Xiao-ping, Yang, Qiao-xin, Yin, Wen, Lei, Ying-feng, Zhang, Fang-lin
Format Journal Article
LanguageChinese
Published China 01.03.2005
Subjects
Animals
Cell Membrane - metabolism
Cercopithecus aethiops
CHO Cells
Cricetinae
Cricetulus
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Epitopes - metabolism
Hantaan virus - immunology
Microscopy, Confocal
Oligopeptides - immunology
Oligopeptides - metabolism
Peptide Library
Vero Cells
Viral Proteins - immunology
Viral Proteins - metabolism
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Summary:To identify and characterize the epitope associated with the virus attachment protein (VAP) of hantaan virus. The monoclonal antibody 3G1 was used as the ligand to biospan from a phage-displayed 12-amino acid peptide library, then the positive phage clones were chosen and sequenced. The amino acid sequences of them were compared with that of hantaan virus G2 in homology. The characteristics of positive phage were studied by IFA and ELISA. A decapeptide combining to cell membrane was observed under laser scanning confocal microscope (LSCM). The conservative motif PX(1-2) HX(0-2) H displaying on positive clones shared homologous amino acid sequence with G2 96YPWHTAKCHY105. G2 96YPWHTAKCHY105 might play some roles in virus binding to host cell, and might be a possible key epitope of hantaan virus VAP.
ISSN:1003-9279