Ischemic postconditioning induces neuroprotection via up-regulation of endogenous antioxidant enzyme activities: experiment with rabbits

• Published in: Zhong hua yi xue za zhi Vol. 88; no. 33; p. 2355
• Main Authors: Song, Wen-ying, Dong, Hai-long, Cheng, Qiong, Lu, Zhi-hong, Wang, Hui, Meng, Jing-ru, Xiong, Li-ze
• Format: Journal Article
• Language: Chinese
• Published: China 26.08.2008
• Subjects: Animals; Antioxidants - metabolism; Catalase - metabolism; Disease Models, Animal; Ischemic Preconditioning - methods; Male; Rabbits; Reperfusion Injury - metabolism; Reperfusion Injury - prevention & control; Spinal Cord Ischemia - metabolism; Spinal Cord Ischemia - prevention & control; Superoxide Dismutase - metabolism; Up-Regulation
• ISSN: 0376-2491

To test the hypothesis that spinal cord protection induced by ischemic postconditioning is mediated by an increase of endogenous antioxidant enzyme activities during reperfusion phase in spinal cord. Seventy-eight male New Zealand rabbits were randomly divided into 3 groups: Sham group (n = 18) undergoing sham operation without aortic occlusion; ischemia/reperfusion (I/R) group (n = 30) undergoing occlusion of the infrarenal abdominal aorta for 20 min, followed by reperfusion; and postconditioning (PostC) group (n = 30) undergoing occlusion of the infrarenal abdominal aorta for 20 min followed by 3 cycles of 30 s reperfusion/30 s ischemia just at the onset of reperfusion. 30 min, and 1, 3, 6, 24, and 48 h after reperfusion 5 rabbits from each group (and 3 from the Sham group) were killed with their spinal cords taken out, and spectrophotometric method was used to determine the antioxidant enzyme activity and malondialdehyde (MDA) content 6, 24, and 48 h after reperfusion motor function scoring of the hind lim