Effect of HBsAg pulsed dendritic cells on the functions of cytokine-induced killer cells

To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells. The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs...

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Published inXi bao yu fen zi mian yi xue za zhi Vol. 21; no. 5; p. 634
Main Authors Liu, Shu-ren, Zhang, Yan, Xie, Qing, Li, Zhuo-liang, Yu, Zhou-yao, Kong, Xiang-ping
Format Journal Article
LanguageChinese
Published China 01.09.2005
Subjects
Cell Line, Tumor
Cell Proliferation
Cytokine-Induced Killer Cells - immunology
Dendritic Cells - immunology
Hepatitis B Surface Antigens - immunology
Hepatitis B Surface Antigens - physiology
Humans
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Summary:To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells. The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The (3)H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells. LDH release assay was used to measure the specific killing activity of CIK cells on HepG2215 cells. The HBsAg-pulsed DCs could induce the memory proliferation of CIK cells and strengthen the killing activity of CIK cells (P<0.05). HBsAg-pulsed DCs can enhance the proliferation and killing functions of CIK cells.
ISSN:1007-8738