Calpain I inhibition prevents pacing-induced structural remodeling for atrial fibrillation in canine
To study the relation of the structural remodeling processes and activation of calpain I. Fifteen dogs were randomly divided into three groups. The dogs in pacing group (n=5) and inhibitor group (n=5) were subjected to 3 weeks of rapid atrial pacing at 600 beats/min, control dogs (n=5) were in sham-...
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Published in | Zhōnghuá xīnxuèguănbìng zázhì Vol. 35; no. 2; p. 132 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.02.2007
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Subjects | |
Online Access | Get more information |
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Summary: | To study the relation of the structural remodeling processes and activation of calpain I.
Fifteen dogs were randomly divided into three groups. The dogs in pacing group (n=5) and inhibitor group (n=5) were subjected to 3 weeks of rapid atrial pacing at 600 beats/min, control dogs (n=5) were in sham-operated group. The dogs in inhibitor group were administered intravenous N-Acetyl-Leu-Leu-Met (ALLM), a calpain inhibitor, and in pacing group and sham-operated group were administered intravenous DMSO. The activity of calpain I was measured by hydrolyzing Suc-Leu-Leu-Val-Tyr-7-amino-4-methyl-coumarin. The ultrastructure of atrium was examined by light and electron microscopy. TnT expression was assessed by Western blot. Echocardiography examination was performed in all the three groups.
Calpain I activity was significantly increased in pacing group (2.3-fold, P<0.01), and decreased in inhibitor group (1.1-fold, P>0.05), compared to sham-operated group respectively. The percentages of myolysis were (76.7 +/- 5.9)% |
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ISSN: | 0253-3758 |