Human mucin 1 promoter drives human sodium/iodide symporter gene targeting expression in pancreatic carcinoma cells

• Published in: Zhong hua yi xue za zhi Vol. 87; no. 39; p. 2780
• Main Authors: Zhou, Quan-bo, Chen, Ru-fu, Li, Zhi-hua, Pan, Qiu-hui, Zhou, Jia-jia, Tang, Qi-bin, Chen, Ji-sheng
• Format: Journal Article
• Language: Chinese
• Published: China 23.10.2007
• Subjects: Base Sequence; Biological Transport; Cell Line, Tumor; Gene Expression Regulation; HeLa Cells; Humans; Iodine Radioisotopes - pharmacokinetics; Molecular Sequence Data; Mucin-1 - genetics; Mucin-1 - metabolism; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Plasmids - genetics; Promoter Regions, Genetic - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Symporters - genetics; Symporters - metabolism; Transfection
• ISSN: 0376-2491

To clone human mucin 1 (MUC1) gene promoter and apply to drive human sodium/iodide symporter (hNIS) gene targeting expression in pancreatic carcinoma cells. Human Mucin1 (MUC1) promoter was cloned from the 5' flanking region of the MUC1 gene by two-step nest PCR from human pancreatic carcinoma cells of the line CAPAN-I, II and then linked to pDC316 plasmid (pDC316-MUC1). Subsequently, a recombinant plasmid containing MUC1 and hNIS was constructed (pDC316-MUC1/hNIS). The recombinant plasmid pDC316-MUC1/hNIS, pD316-mCMV/NIS plasmid, and pDC316-mCMV/hNIS plasmid were transfected into the CAPAN-II cells, human pancreatic carcinoma cells of the line PANC-1, and human cervical carcinoma cells of the line HeLa respectively as experimental group, positive control group, and negative control group. 48 h after the transfection RT-PCR and immunofluorescence were used to confirm the expression of hNIS mRNA and hNIS protein. Then the cells were cultured in solution with 125I. The 125I uptake in the cells was measured by g