Relationship between the expression of heat shock protein 25 and selenium binding protein 1 with ischemia/reperfusion lung injury in vivo: experiment with rats

• Published in: Zhong hua yi xue za zhi Vol. 88; no. 27; p. 1929
• Main Authors: Liu, Jian-xin, Wang, Dian-jun, Zhang, Qing-chun, Yin, Bang-liang, Yang, Jin-fu, Hu, Jian-guo
• Format: Journal Article
• Language: Chinese
• Published: China 15.07.2008
• Subjects: Animals; Electrophoresis, Gel, Two-Dimensional; HSP27 Heat-Shock Proteins - analysis; HSP27 Heat-Shock Proteins - metabolism; Lung - blood supply; Lung - metabolism; Male; Proteome - analysis; Proteome - metabolism; Proteomics - methods; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury - metabolism; Reperfusion Injury - physiopathology; Selenium-Binding Proteins - analysis; Selenium-Binding Proteins - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0376-2491

To analyze the differential expression proteins of rat ischemia/reperfusion (I/R) lung tissues in vivo and normal lung tissues by comparative proteome analysis, and to study the mechanism of donor lung I/R injury. Forty male SD rats were randomly divided into 2 equal groups: I/R group undergoing mimic orthotopic left lung auto-grafting and harvesting of the left lung five hours after the operation, and control group undergoing isolation of the left hilus of lung and then harvesting of the left lung. The differential proteins in the left ventricle of transplanted heart were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE), identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and searched through Matrix Science software system. Western blotting was used to verify part of the differentially expressed proteins. Well-resolved and reproducible 2-DE profile of rat I/R lung tissues and normal lung tissues were obtained