Comparison of modification of surface xenoantigens on bovine and porcine erythrocytes

This study was aimed to explore impact of removal of cell membrane G alalpha1-3Gal beta1-4Glc NAc epitopes (called alpha-Gal) and chemical modification of other xenoantigen on bovine red blood cell (bRBC) and porcine red blood cell (pRBC) antigenicity and to compare their modified erythrocytes, in o...

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Bibliographic Details
Published inZhongguo shi yan xue ye xue za zhi Vol. 13; no. 5; p. 878
Main Authors Tan, Ying-Xia, Li, Su-Bo, Wang, Jie-Xi, Zhang, Yang-Pei
Format Journal Article
LanguageChinese
Published China 01.10.2005
Subjects
alpha-Galactosidase - immunology
Animals
Antigens, Heterophile - immunology
Blood Substitutes
Cattle
Disaccharides - immunology
Epitopes - immunology
Erythrocyte Membrane - immunology
Erythrocyte Transfusion - methods
Erythrocytes - immunology
Erythrocytes - metabolism
Humans
Swine
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Summary:This study was aimed to explore impact of removal of cell membrane G alalpha1-3Gal beta1-4Glc NAc epitopes (called alpha-Gal) and chemical modification of other xenoantigen on bovine red blood cell (bRBC) and porcine red blood cell (pRBC) antigenicity and to compare their modified erythrocytes, in order to provide basis for development of human blood substitute with rich source, high safety and efficacy. bRBC and pRBC were subjected to both enzymatic removal of membrane alpha-Gal with recombinant coffee bean alpha-galactosidase (rC alpha-GalE) and covalent attachment of benzotriazole carbonate-linked methoxypolyethylene glycol (mPEG-BTC, MW = 20 kD). The effects of treatment were measured by hemagglutination, flow cytometric assay of IgG binding and clinical cross-match testing to human sera. The results showed that although alpha-galactosidase treatment reduced hemagglutination titers to levels similar to negative control, the combination of the treatments was most effective. Clinically used cross-match test
ISSN:1009-2137