Effects of Salviae miltiorrhizae on H2O2-induced apoptosis of rat vascular smooth muscle cells

To investigate the effects of Salviae miltiorrhizae on H2O2-induced apoptosis in rat vascular smooth muscle cells (VSMC(s)) and its related mechanism. The 3-5 passages of VSMC(s) were used, H2O2 was added to the medium of cultured rat aortic VSMC(s) as extraneous reactive oxygen species (ROS). The v...

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Bibliographic Details
Published inZhong yao cai Vol. 27; no. 9; p. 659
Main Authors Du, Xianhua, Xiang, Hu, Yu, Longshun, Yang, Fangju
Format Journal Article
LanguageChinese
Published China 01.09.2004
Subjects
Animals
Aorta
Apoptosis - drug effects
Cells, Cultured
Drugs, Chinese Herbal - pharmacology
Flow Cytometry
Hydrogen Peroxide
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Rats
Rats, Wistar
Salvia miltiorrhiza
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Summary:To investigate the effects of Salviae miltiorrhizae on H2O2-induced apoptosis in rat vascular smooth muscle cells (VSMC(s)) and its related mechanism. The 3-5 passages of VSMC(s) were used, H2O2 was added to the medium of cultured rat aortic VSMC(s) as extraneous reactive oxygen species (ROS). The viability percentage of VSMC(s) was evaluated by MTT colorimetric assay. The percentage of apoptosis was measured by flow cytometry. The expression of Bax/Bcl-2 protein was examined with immunohistochemical technique and positive index (PI) was calculated. 1 mmol/L H2O2 could increase apoptosis rate. The PI of Bax/Bcl-2 protein in H2O2 group was higher (P < 0.01). When VSMC(s) were incubated with H2O2 plus Salviae miltiorrhizae, the apoptosis rate greatly declined, and at the same time, the PI of Bax/Bcl-2 protein reduced remarkably compared with H2O2 group (P < 0.05 or P < 0.01). Salviae miltiorrhizae could prevent VSMC(s) from H2O2-induced apoptosis by decreasing the Bax/Bcl-2 protein expression.
ISSN:1001-4454