Antiviral effect of human CTLs activated by HBsAg stimulated dendritic cells in vitro

To investigate the ability of human dendritic cells (DCs) inducing specific T lymphocyte response and inhibit the expression of HBeAg and HBsAg in 2.2.15 cell culture supernatant. DCs were prepared from peripheral blood mononuclear cells induced with granulocyte macrophage colony-stimulating factor(...

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Published inZhonghua shi yan he lin chuang bing du xue za ji Vol. 17; no. 4; p. 365
Main Authors Xing, Li-he, Wang, Fu-sheng, Zhu, Chuan-lin
Format Journal Article
LanguageChinese
Published China 01.12.2003
Subjects
Adolescent
Adult
Antigen Presentation
Cells, Cultured
Child
Dendritic Cells - drug effects
Dendritic Cells - immunology
Female
Hepatitis B Surface Antigens - pharmacology
Hepatitis B, Chronic - immunology
Humans
Lymphocyte Activation
Male
Middle Aged
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Summary:To investigate the ability of human dendritic cells (DCs) inducing specific T lymphocyte response and inhibit the expression of HBeAg and HBsAg in 2.2.15 cell culture supernatant. DCs were prepared from peripheral blood mononuclear cells induced with granulocyte macrophage colony-stimulating factor(GM-CSF) and interleukin 4. DCs was impulsed with pure HBsAg before DCs maturation and cocultured with self-blood T lymphocyte, while DCs without pure HBsAg stimulated group, T lymphocyte group and only T lymphocyte group were prepared as control group. The culture supernatant of 2.2.15 cell with stimulated T lymphocytes was collected on day 1, day 3, day 5 and day 7, respectively. The expressed levels of HBeAg and HBsAg were detected by ELISA method. DCs after antigen stimulation had a strong ability to present antigen and induce immune activation, DCs after loading with antigen in normal control and chronic hepatitis patients group had stronger stimulative ability for T lymphocytes proliferation than that of DCs w
ISSN:1003-9279