A study of T-cell reconstitution after HLA-matched sibling bone marrow transplantation in leukemia patients

To study T-cell reconstitution by the assessment of T-cell receptor excision circle (TRECs) and T-cell receptor clonal repertoire after HLA-matched sibling bone marrow transplantation (MSD-BMT) in leukemia patients and try to reveal the rule of T-cells repopulation in MSD-BMT. Real-time quantitative...

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Published inChung-hua nei kʿo tsa chih Vol. 46; no. 9; p. 743
Main Authors Fu, Yue-Wen, Wu, De-Pei, Cen, Jian-Nong, Feng, Yu-Feng, Zhu, Zi-Ling, Zhu, Ping
Format Journal Article
LanguageChinese
Published China 01.09.2007
Subjects
Adolescent
Adult
Bone Marrow Transplantation - immunology
Bone Marrow Transplantation - methods
Female
Histocompatibility Testing
Humans
Leukemia - genetics
Leukemia - immunology
Leukemia - surgery
Living Donors
Lymphocyte Depletion
Male
Middle Aged
Receptor-CD3 Complex, Antigen, T-Cell - biosynthesis
Receptor-CD3 Complex, Antigen, T-Cell - genetics
Reverse Transcriptase Polymerase Chain Reaction
Siblings
T-Lymphocytes - immunology
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Summary:To study T-cell reconstitution by the assessment of T-cell receptor excision circle (TRECs) and T-cell receptor clonal repertoire after HLA-matched sibling bone marrow transplantation (MSD-BMT) in leukemia patients and try to reveal the rule of T-cells repopulation in MSD-BMT. Real-time quantitative PCR detection of TRECs was carried out in the DNA of peripheral blood mononuclear cells from 23 leukemia patients who underwent MSD-BMT. The content of TRECs in 70 normal donor individuals was also detected to determine the normal range of TRECs in healthy subjects. Among them, 10 patients received RT-PCR to amplify 24 subfamily genes of T-cell receptor B variable (TCRBV) and five normal donors served as control. The PCR products were further analyzed with genescan to evaluate the clonality of BV subfamily and calculate the usage rate of BV families. The mean value of TRECs in normal donors was (3351.1 +/- 3711.1) copies/10(5) cells. There was an inverse correlation between the value of TRECs and the age of health
ISSN:0578-1426