Antitumor activity of zinostatin stimalamer (YM881) in human hepatoma cell lines and VX2 liver tumor-bearing rabbits

• Published in: Research communications in molecular pathology and pharmacology Vol. 92; no. 2; p. 155
• Main Authors: Tanaka, S, Fujihara, A, Yamamoto, M, Ida, H, Ohmi, Y, Kitazaki, T, Kikuchi, Y, Yoshida, S, Okamiya, H, Numasaki, Y, Konno, T
• Format: Journal Article
• Language: English
• Published: United States 01.05.1996
• Subjects: Animals; Antineoplastic Agents - pharmacology; Carcinoma, Hepatocellular - drug therapy; Cell Division - drug effects; Disease Models, Animal; Drug Screening Assays, Antitumor; Humans; Iodized Oil - pharmacology; Liver Neoplasms - drug therapy; Liver Neoplasms, Experimental - drug therapy; Male; Maleic Anhydrides - pharmacology; Neoplasm Transplantation; Polystyrenes - pharmacology; Rabbits; Suspensions; Tumor Cells, Cultured; Zinostatin - analogs & derivatives; Zinostatin - pharmacology
• ISSN: 1078-0297

Antitumor activities of zinostatin stimalamer (YM881) were examined in human hepatoma cell lines (SK-Hep1 and HuH2) and VX2 liver tumor-bearing rabbits. YM881 inhibited the growth of human hepatoma cells in a dose-dependent manner. The IC50 values of YM881 causing a 50% inhibition of growth of SK-Hep1 and HuH2 cells were 6.7 and 27 nM, respectively. In VX2 tumor-bearing rabbits, administration of YM881 suspended in Lipiodol, an iodinated fatty acid ethylester of poppyseed oil, (YM881/Lipiodol suspension, 0.2 mg/0.2 ml/body) into the hepatic artery showed significant (p < 0.01, vs. sham-operated and Lipiodol-treated groups) inhibitory effects on tumor growth and histopathological changes at 1 and 2 weeks after administration. In contrast, Lipiodol (0.2 ml/body) tended to inhibit the growth of VX2 tumor (p < 0.1, vs. sham-operated group) at 1 week after administration, but showed only moderate effects at 2 weeks after administration. Minimal necrosis was observed at 1 and 2 weeks after administration of Lipiodol, and histopathological findings were similar to those in the sham-operated group. From the present study, it is suggested that YM881/Lipiodol suspension showed antitumor activity in VX2 tumor-bearing rabbits presumably due to the inhibition of the growth of hepatoma cells by YM881 itself. Lipiodol, on the other hand, is considered to augment the antitumor activity of YM881 by maintaining high YM881 concentrations in tumor tissue.