Comparative effect of osmium tetroxide and ruthenium tetroxide on Penicillium sp. hyphae and Saccharomyces cerevisiae fungal cell wall ultrastructure

The fungal cell wall viewed through the electron microscope appears transparent when fixed by the conventional osmium tetroxide method. However, ruthenium tetroxide post-fixing has revealed new details in the ultrastructure of Penicillium sp. hyphae and Saccharomyces cerevisiae yeast. Most significa...

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Bibliographic Details
Published inBiomédica Vol. 23; no. 2; pp. 225 - 231
Main Authors Torres-Fernández, Orlando, Ordóñez, Nelly
Format Journal Article
LanguageEnglish
Published Colombia 01.06.2003
Subjects
Cell Wall - ultrastructure
Humans
Osmium Tetroxide - pharmacology
Penicillium - drug effects
Penicillium - ultrastructure
Ruthenium Compounds - pharmacology
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - ultrastructure
Tissue Fixation
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Summary:The fungal cell wall viewed through the electron microscope appears transparent when fixed by the conventional osmium tetroxide method. However, ruthenium tetroxide post-fixing has revealed new details in the ultrastructure of Penicillium sp. hyphae and Saccharomyces cerevisiae yeast. Most significant was the demonstration of two or three opaque diverse electron dense layers on the cell wall of each species. Two additional features were detected. Penicillium septa presented a three-layered appearance and budding S. cerevisiae yeast cell walls showed inner filiform cell wall protrusions into the cytoplasm. The combined use of osmium tetroxide and ruthenium tetroxide is recommended for post-fixing in electron microscopy studies of fungi.
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content type line 23
ISSN:0120-4157
DOI:10.7705/biomedica.v23i2.1215