A study on the inactivation of p16 genes and the expression of P16 protein in primary hepatocellular carcinomas

• Published in: Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Vol. 31; no. 3; p. 306
• Main Authors: Huang, J, Shen, W, Li, B, Luo, Y, Liao, S, Zhang, W, Cheng, N
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2000
• Subjects: Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Cyclin-Dependent Kinase Inhibitor p16 - genetics; Cyclin-Dependent Kinase Inhibitor p16 - metabolism; Gene Deletion; Genes, p16; Humans; Immunohistochemistry; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational
• ISSN: 0257-7712

The aim of the present study was to determine whether p16 gene is involved in the genesis of primary hepatocellular carcinoma (HCC). Twenty-five primary HCC tumor and corresponding non-tumor liver tissue specimens were examined for P16 gene alterations. The identification of deletion of p16 gene exon 1 and exon 2 was performed using comparative multiplex polymerase chain reaction (PCR) analysis. The point mutation of p16 gene exon 2 was investigated by single strand conformational polymorphism(SSCP) analysis, and the status of p16 gene methylation was screened using PCR-based methylation analysis. Moreover, 35 parafin specimens of primary HCCs with corresponding non-tumor liver tissues, including the 25 cases described above for screening of p16 gene alterations, were investigated also for P16 protein expression by using immunohistochemical analysis. The results of comparative multiplex PCR analysis showed that 12% (3/25) p16 gene deletions, including homozygous deletions in 2 tumors and hemizygous deletion i