Immunohistochemical localization and expression of fibromodulin in the periodontium

• Published in: Zhong hua yi xue za zhi Vol. 83; no. 19; p. 1682
• Main Authors: Qian, Hong, Fan, Ming-wen, Xiao, Y, Bartold, P M
• Format: Journal Article
• Language: Chinese
• Published: China 10.10.2003
• Subjects: Animals; Biglycan; Carrier Proteins - analysis; Carrier Proteins - genetics; Collagen - analysis; Collagen - genetics; Decorin; Extracellular Matrix Proteins; Fibromodulin; Immunohistochemistry; Male; Periodontium - chemistry; Proteoglycans - analysis; Proteoglycans - genetics; Rats; Rats, Inbred Lew; Reverse Transcriptase Polymerase Chain Reaction
• ISSN: 0376-2491

To study the distribution and expression of fibromodulin in normal periodontium, so as to understand its role in periodontal tissue homeostasis. Five normal male Lewis rats were killed and their bilateral mandibular first molars with surrounding alveolar bones and gingival tissues were taken out. Human gingival fibroblasts, fibroblasts of periodontal ligament, and osteoblasts were cultured. Immunohistochemistry with anti-fibromodulin, anti-decorin, anti-biglycan, anti-type I collagen, and anti-type III collagen antibodies and RT-PCR were used to detect the tissue distribution and cellular localization of fibromodulin and related proteoglycans, decorin and biglycan, and type I and type III collagens. Fibromodulin was strongly expressed in the suprabasal gingival epithelium, periodontal ligament, alveolar bone, gingival and periodontal fibroblasts as well as their matrices. Strong expression was noted in the area close to oral gingival aspect and the interfaces of periodontal ligament-alveolar bone and periodon