Cyclin B1 and CDK1: nuclear localization and upstream regulators

Formation of an active nuclear cyclin B1-CDK1 complex is a highly intricate procedure requiring many different levels of regulation. Each of these regulatory steps represents a potential target for controlling cell proliferation. Accumulation of threshold levels of cyclin B1 protein at the G2/M tran...

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Bibliographic Details
Published inProgress in cell cycle research Vol. 5; p. 335
Main Authors Porter, Lisa A, Donoghue, Daniel J
Format Journal Article
LanguageEnglish
Published France 2003
Subjects
Active Transport, Cell Nucleus - drug effects
Active Transport, Cell Nucleus - genetics
Animals
Antineoplastic Agents - pharmacology
CDC2 Protein Kinase - drug effects
CDC2 Protein Kinase - genetics
CDC2 Protein Kinase - metabolism
Cell Cycle - drug effects
Cell Cycle - physiology
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cyclin B - drug effects
Cyclin B - genetics
Cyclin B - metabolism
Cyclin B1
Drug Evaluation, Preclinical
Humans
Phosphorylation - drug effects
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Summary:Formation of an active nuclear cyclin B1-CDK1 complex is a highly intricate procedure requiring many different levels of regulation. Each of these regulatory steps represents a potential target for controlling cell proliferation. Accumulation of threshold levels of cyclin B1 protein at the G2/M transition requires the cooperation of various promoter elements, possibly the activation of several transcription factors, enhanced cyclin B1 mRNA stability and, in some cases, translational activation of dormant mRNA. Binding of cyclin B1 to its inactive partner, CDK1, initiates conformational changes allowing CDK1 to alter its phosphorylation status and to become an active kinase. Lastly, the active cyclin B1-CDK1 complex must translocate to the nucleus to begin phosphorylating nuclear substrates. These phosphorylation events are necessary for mitotic onset. While cyclin B1 is capable of shuttling from the nucleus to the cytoplasm throughout interphase, mitotic onset requires phosphorylation of cyclin B1 within the CRS region, thereby enhancing import and inhibiting export of the cyclin B1-CDK1 complex. Elucidating the role of mediators controlling cyclin B1-CDK1 translocation at the onset of mitosis is essential in developing drug targets for cell cycle control.
ISSN:1087-2957