Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides we...

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Published inNucleic acids symposium series (1979) no. 44; p. 87
Main Authors Masaoka, A, Terato, H, Honsho, A, Ohyama, Y, Suzuki, T, Yamada, M, Makino, K, Ide, H
Format Journal Article
LanguageEnglish
Published England 2000
Subjects
Deoxyribonucleotides - metabolism
DNA Damage
DNA Glycosylases
DNA Repair
Guanine - metabolism
In Vitro Techniques
N-Glycosyl Hydrolases - metabolism
Nitric Oxide - toxicity
Nitrogen Dioxide - toxicity
Nucleotides - metabolism
Purine Nucleosides - metabolism
Pyrroles - metabolism
Xanthine - metabolism
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Summary:Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
ISSN:0261-3166