Polymorphisms of five short tandem repeat systems in Chinese Zang population in Kangba area

To get preliminary genotype and allele frequency distributions of D1S549, D3S1754, D12S391, D12S375 and D18S865 loci in Chinese Zang (Tibetan) population in Kangba area and to validate more short tandam repeat (STR) systems for forensic application. Fresh samples of venous blood from 100 unrelated i...

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Bibliographic Details
Published inSichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 35; no. 1; p. 21
Main Authors Hu, Yu, Liao, Miao, Zhou, Bin, Jia, Yi, Zhang, Lin, Chen, Guo-di
Format Journal Article
LanguageChinese
Published China 01.01.2004
Subjects
Alleles
China - ethnology
Electrophoresis, Polyacrylamide Gel
Gene Frequency
Genetic Markers - genetics
Humans
Polymerase Chain Reaction
Polymorphism, Genetic
Tandem Repeat Sequences - genetics
China
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Summary:To get preliminary genotype and allele frequency distributions of D1S549, D3S1754, D12S391, D12S375 and D18S865 loci in Chinese Zang (Tibetan) population in Kangba area and to validate more short tandam repeat (STR) systems for forensic application. Fresh samples of venous blood from 100 unrelated individuals of Chinese Zang population in Kangba area were dropped on the filter paper. After they dried up, DNA were extracted by chelex-100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed by PAG vertical electrophoresis and silver staining. Seven alleles were found at D1S549 locus, six alleles at D3S1754 locus, ten alleles at D12S391 locus, six alleles at D12S375 and six alleles at D18S865 locus. No deviations from Hardy-Weinberg equilibrium were observed. The heterozygosities observed were 0.86, 0.77, 0.81, 0.69, 0.80 for D1S549, D3S1754, D12S391, D12S375 and D18S865 respectively. The calculated values of the Power of Exclusion were 0.715, 0.545, 0.618, 0.413 and 0.599,
ISSN:1672-173X