Establishment of dextran sulfate sodium-induced ulcerative colitis model in mice

To establish a model of ulcerative colitis in mice. The mice were given 5% dextran sulfate sodium (DSS) solution freely for 7 consecutive days after which distilled water was given in stead for another 10 d, to complete one cycle of whole treatment plan that consisted of 4 such cycles. The symptoms...

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Bibliographic Details
Published inDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Vol. 22; no. 7; p. 608
Main Authors Wang, Qun-Ying, Chen, Cun-Long, Wang, Ji-De, Lai, Zhuo-Sheng, Liu, Rong, Zhang, Ya-Li
Format Journal Article
LanguageEnglish
Published China 01.07.2002
Subjects
Animals
Colitis, Ulcerative - chemically induced
Dextran Sulfate
Disease Models, Animal
Female
Male
Mice
Mice, Inbred BALB C
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Summary:To establish a model of ulcerative colitis in mice. The mice were given 5% dextran sulfate sodium (DSS) solution freely for 7 consecutive days after which distilled water was given in stead for another 10 d, to complete one cycle of whole treatment plan that consisted of 4 such cycles. The symptoms were observed daily. At the end of the first and the fourth cycle respectively, the mice were killed for examining the whole colon under anatomic microscopy and serial tissue sections were prepared for histological observation. The symptoms observed in the mice including hematochezia diarrhea and loss of the body weight were similar to those of ulcerative colitis patients. Histological examination revealed infiltration of neutrocytophilia and lymphocythemia, with loss of integrity of the colon mucosa in the gland. This mouse model of ulcerative colitis can be easily induced and readily applied in various studies of ulcerative colitis.
ISSN:1000-2588