Study on using colloidal gold immuno-dot assay to detect special antibody of hemorrhagic fever renal syndrome

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 21; no. 7; p. 504
• Main Authors: Chu, F, Ji, Q, Yan, R M
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2001
• Subjects: Adolescent; Adult; Aged; Antibodies, Viral - blood; Drug Therapy, Combination; Drugs, Chinese Herbal - therapeutic use; Female; Gold Colloid; Hantaan virus - immunology; Hemorrhagic Fever with Renal Syndrome - drug therapy; Hemorrhagic Fever with Renal Syndrome - immunology; Humans; Immunoassay - methods; Immunoglobulin G - blood; Immunoglobulin M - blood; Male; Middle Aged; Phytotherapy; Ribavirin - therapeutic use
• ISSN: 1003-5370

To seek for a simple, sensitive and rapid assay to detect specific antibody of patients suffering from hemorrhagic fever renal syndrome (HFRS). Serum anti-HFRS IgM (sIgM) and IgG (sIgG) antibody were detected in 186 patients with HFRS using colloidal gold immuno-dot assay (CGIDA), and compared with those detected with enzyme linked immunosorbant assay (ELISA) and indirect fluorescent antibody test (IFAT). Kuhuang Shenmai injection (KHSM) combined Astragalus decoction (AD) treatment was applied to 101 HFRS patients in a treated group (n = 50), and the effect of therapy was compared with that in a control group (n = 51) treated with ribarvirin and ganlixin injection. CGIDA showed positive sIgM antibody in 132 cases (70.9%) and positive sIgG antibody in 163 (87.1%) of the 186 HFRS patients. As compared the two groups in crossing stage, the rate of crossing shock stage in the treated group was higher than that in the control (P < 0.05). As for rate of crossing oliguria stage and from febrile stage directly develo