Mapping of BRCT1 domain of BRCA1 with chromatin unfolding activity

Breast cancer susceptibility gene 1(BRCA1) plays an important role in breast cancer development and progression. BRCA1 encodes a 1863-amino acid protein with two BRCA1 C-terminal (BRCT) domains at its C-terminus, BRCT1 and BRCT2. Many cancer-predisposing mutations are located in the BRCT domains, wh...

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Bibliographic Details
Published inShengwu gongcheng xuebao Vol. 18; no. 6; p. 656
Main Authors Ye, Qi-Nong, Hu, Yan-Fen, Zhong, Hong-Jun, Li, Rong, Huang, Cui-Fen
Format Journal Article
LanguageChinese
Published China 01.11.2002
Subjects
Base Sequence
BRCA1 Protein - chemistry
BRCA1 Protein - physiology
Chromatin - chemistry
Cloning, Molecular
Female
Genes, BRCA1
Humans
Molecular Sequence Data
Mutation
Protein Folding
Structure-Activity Relationship
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Summary:Breast cancer susceptibility gene 1(BRCA1) plays an important role in breast cancer development and progression. BRCA1 encodes a 1863-amino acid protein with two BRCA1 C-terminal (BRCT) domains at its C-terminus, BRCT1 and BRCT2. Many cancer-predisposing mutations are located in the BRCT domains, which have been shown to induce chromatin unfolding by use of an approach that allows visualization of large-scale chromatin structure through lac repressor/lac operator recognition. To map the important region of BRCT domain (amino acid residues 1642-1736), six deletion mutant constructs were made. The chromatin structure assay showed that amino acid residues 1691-1721 are involved in the induction of chromatin unfolding. To further localize the critical amino acid residues, ten alanine scanning mutant constructs were made. The chromatin structure assay demonstrated that the 1707IAGGK1711 region is critical for the chromatin unfolding activity. Based on the mapped important region, Blast analysis identified a novel
ISSN:1000-3061