Influences of porcine enamel matrix proteins on MC3T3-E1 osteoblast proliferation and differentiation

Some research suggested that enamel matrix proteins(EMPs) not only induced formation of cementum, but also could promote regeneration of periodontal tissue and alveolar bone. In order to recognize the mechanisms of periodontal regeneration induced by EMPs and provide a basic data for further study,...

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Published inHua xi kou qiang yi xue za zhi Vol. 18; no. 4; p. 226
Main Authors Shu, R, Liu, Z, Ge, L
Format Journal Article
LanguageChinese
Published China 01.08.2000
Subjects
Animals
Cell Differentiation - drug effects
Cell Division - drug effects
Cell Line
Dental Enamel Proteins - pharmacology
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - physiology
Swine
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Summary:Some research suggested that enamel matrix proteins(EMPs) not only induced formation of cementum, but also could promote regeneration of periodontal tissue and alveolar bone. In order to recognize the mechanisms of periodontal regeneration induced by EMPs and provide a basic data for further study, influences of EMPs on osteoblast proliferation and differentiation are explored in this study. Porcine enamel matrix proteins were extracted by using acetic acid. MC3T3-E1 osteoblasts were selected and cell culture was used in vitro. EMPs with different concentrations of 50 micrograms/ml, 100 micrograms/ml and 150 micrograms/ml were added separately in alpha-MEM culture medium and compared with negative group and positive group (TGF-beta 1). The count of cell was recorded after 0, 1, 2, 3, 4, 5, 6 days, then the data were analysed statistically by using SAS. The cell count of all EMPs groups were higher than that in the negative control group (P < 0.01), 100 micrograms/ml as well as 150 micrograms/ml EMPs groups we
ISSN:1000-1182