Influence of nutritional supplements on keratinolysis by Amycolatopsis keratinophila

Keratinolytic potential of A. keratinophila (DSM 44409T), a newly described Amycolatopsis sp. isolated from cultivated soil in Kuwait, was demonstrated using keratinazure as the sole source of carbon and nitrogen as estimated by gel diffusion assay. Effects of 12 various nutritional supplements on t...

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Published inIndian journal of experimental biology Vol. 41; no. 8; pp. 870 - 874
Main Authors Al-Musallam, A A, Al-Zarban, S S, Al-Sarawi, H K, Kroppenstedt, R M, Stackebrandt, E, Fasasi, Y A
Format Journal Article
LanguageEnglish
Published India 01.08.2003
Subjects
Actinomycetales - enzymology
Actinomycetales - growth & development
Biomass
Carbon
Culture Media
Endopeptidases - biosynthesis
Endopeptidases - isolation & purification
Keratins - metabolism
Nitrogen
Peptide Hydrolases - biosynthesis
Peptide Hydrolases - isolation & purification
Sepharose - metabolism
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Summary:Keratinolytic potential of A. keratinophila (DSM 44409T), a newly described Amycolatopsis sp. isolated from cultivated soil in Kuwait, was demonstrated using keratinazure as the sole source of carbon and nitrogen as estimated by gel diffusion assay. Effects of 12 various nutritional supplements on the keratinolytic and azocollytic activities were determined. NH4H2PO4 and KNO3 in the medium supported a significantly higher keratinolytic activity than other supplements. However, azocollytic activities in all the supplemented media and the control were same. Best combination of carbon and nitrogen supplements (galactose and NH4H2PO4 respectively) used to evaluate the dynamics of growth and enzymes (keratinase and protease) activities of the isolate revealed a luxuriant growth with optimal keratinolytic activity occurring during the log phase. Other parameters of the fermentation medium, including pH, biomass accumulation, total protein and free amino acid concentrations were also studied.
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ISSN:0019-5189