Clinical and experimental study of two cases of myelodysplastic syndrome with t(3; 5) (q25; q34) translocation

To report two myelodysplatic syndromes (MDS) patients with t(3; 5) (q25; q34). Chromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was performed by R banding technique, chromosome painting (fluorescence in situ hybridization, FISH) by using whole chromos...

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Published inZhōnghuá xuèyèxué zázhì Vol. 23; no. 6; p. 304
Main Authors Wu, Yafang, Xue, Yongquan, Zhao, Meng, Chen, Saijuan, Pan, Jinlan, Lu, Dingwei
Format Journal Article
LanguageEnglish
Published China 01.06.2002
Subjects
Adolescent
Adult
Antigens, CD - analysis
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 5
Humans
In Situ Hybridization, Fluorescence - methods
Leukocytes, Mononuclear - classification
Leukocytes, Mononuclear - immunology
Male
Myelodysplastic Syndromes - genetics
Myelodysplastic Syndromes - physiopathology
Translocation, Genetic
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Summary:To report two myelodysplatic syndromes (MDS) patients with t(3; 5) (q25; q34). Chromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was performed by R banding technique, chromosome painting (fluorescence in situ hybridization, FISH) by using whole chromosome 3 and 5 probes in case 1. The clinical and hematological findings were compatible with diagnosis of MDS. Karyotype analysis showed that both patients had identical t(3; 5) (q25; q34) translocation. A reciprocal translocation between chromosomes 3q and 5q was proved by FISH in one patient. t(3; 5) translocation is a rare chromosome abnormality specifically associated with MDS and frequently displays trilineage dysplasia. Chromosome painting technique is a reliable tool for detecting this translocation.
ISSN:0253-2727