Determination of clonidine in rabbit plasma by HPLC-MS

• Published in: Yao hsüeh hsüeh pao Vol. 39; no. 5; p. 367
• Main Authors: Ke, Guang-ming, Zhang, En-hong, Wang, Li, Zhang, Qiang, Du, Hong-guang, Guo, Hong-you
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2004
• Subjects: Administration, Cutaneous; Animals; Antihypertensive Agents - administration & dosage; Antihypertensive Agents - blood; Antihypertensive Agents - pharmacokinetics; Area Under Curve; Chromatography, High Pressure Liquid - methods; Clonidine - administration & dosage; Clonidine - blood; Clonidine - pharmacokinetics; Rabbits; Spectrometry, Mass, Electrospray Ionization - methods
• ISSN: 0513-4870

To determine clonidine in rabbit plasma by LC-MS. The LC-MS system consisted of Waters Alliance 2790 HPLC and Micromass ZQ-4000 MS. The HPLC was performed by using XTerra C18 (150 mm x 2.1 mm ID, 5 microm). The mobile phase, consisting of acetonitrile/ammonium hydrogen carbonate solution, was maintained to a flow-rate of 0.2 mL x min(-1) and the linear gradient elution was adopted. Mass spectrum was obtained by using electrospray ionization interface and the m/z of SIM was 230. The average recovery was high and the method was reproducible. The calibration curve showed good linearity in the range of 1 - 80 microg x L(-1), the lowest limit of detection was 0.05 microg x L(-1). The Cmax, AUC0-t, and Tmax value of the pharmacokinetics parameter were (27 +/- 9) microg x L(-1), (5,352 +/- 1,121) microg x L(-1), (79 +/- 17) h. The results demonstrated that the method had high sensitivity, good selectivity, accuracy and precision. It is used to determine the clonidine concentration in plasma. The transdermal patch ca