A study of injectable autogenous tissue-engineered bone

To investigate the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection. Bone marrow cells harvested from iliac bone of New Zealand rabbits were induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mix...

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Bibliographic Details
Published inZhonghua kouqiang yixue zazhi Vol. 38; no. 5; p. 393
Main Authors Yang, Wei-dong, Cao, Qiang, Qian, Qi-chun, Tang, Li-hui, Yang, Lian-jia, Mao, Tian-qiu
Format Journal Article
LanguageChinese
Published China 01.09.2003
Subjects
Alginates - administration & dosage
Animals
Glucuronic Acid - administration & dosage
Hexuronic Acids - administration & dosage
Osteoblasts - transplantation
Osteogenesis
Rabbits
Tissue Engineering
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Summary:To investigate the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection. Bone marrow cells harvested from iliac bone of New Zealand rabbits were induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mixed with 1.5% alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 4 x 10(9)/L. Calcium chloride was used as cross-linking agent to gel aqueous alginate solution. The marrow stromal osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of rabbits with autogenous cells transplantation. The samples were examined with X-ray and histological analysis. Four, eight and twelve weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals. On X-ray photograph the samples showed calcified image with more density than surrounding soft tissue, new bone formation was observed in the osteoblasts/alginate composites in hist
ISSN:1002-0098