Applying competitive polymerase chain reaction to the detection of hepatitis B virus DNA

To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples. A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DN...

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Bibliographic Details
Published inSichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 35; no. 6; p. 858
Main Authors Wang, Ling, Yang, Peng, Li, Shuang-qing, Xu, Shu-hui, Cao, Gui-qun, Zhang, Fa-qiang, Zhang, Mei-xia, Chen, Qing-ying, Xia, Qing-jie, Liu, Kai, Tang, Fang, Zhang, Yuan-zheng
Format Journal Article
LanguageChinese
Published China 01.11.2004
Subjects
DNA, Viral - blood
False Negative Reactions
Hepatitis B - diagnosis
Hepatitis B - virology
Hepatitis B virus - genetics
Humans
Polymerase Chain Reaction - methods
Sensitivity and Specificity
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Summary:To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples. A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DNA. In a 20 microl C-PCR system, about 60 to 200 copies of inner control DNA could give apparent co-amplification signal band after electrophoresis on a 2% agarose gel. Five of 120 samples of clinical serum (4.2%) could not be amplified. C-PCR has the advantage of yielding information on false negative in the HBV DNA PCR assay of clinical serum samples.
ISSN:1672-173X