Monitoring of the therapeutic response of interferon to chronic myeloid leukemia by duel-color fluorescence in situ hybridization

To investigate the significance of duel-color fluorescence in situ hybridization (D-FISH) in monitoring the response to interferon alpha (IFN-alpha) therapy in patients with chronic myeloid leukemia (CML), the D-FISH method was employed to detect the proportion of the interphase nuclei cells with bc...

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Published inZhongguo shi yan xue ye xue za zhi Vol. 11; no. 1; p. 38
Main Authors Song, Yan-Qiu, Li, Wei, Kong, Ling-Hua, Wang, Guan-Jun
Format Journal Article
LanguageChinese
Published China 01.02.2003
Subjects
Adolescent
Adult
Aged
Antineoplastic Agents - therapeutic use
Bone Marrow Cells - drug effects
Bone Marrow Cells - metabolism
Bone Marrow Cells - pathology
Female
Fusion Proteins, bcr-abl - genetics
Humans
In Situ Hybridization, Fluorescence - methods
Interferon-alpha - therapeutic use
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology
Male
Middle Aged
Philadelphia Chromosome
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Treatment Outcome
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Summary:To investigate the significance of duel-color fluorescence in situ hybridization (D-FISH) in monitoring the response to interferon alpha (IFN-alpha) therapy in patients with chronic myeloid leukemia (CML), the D-FISH method was employed to detect the proportion of the interphase nuclei cells with bcr/abl fusion gene in the bone marrow of patients with CML before and after IFN-alpha therapy, and the results were compared with those of bcr/abl fusion mRNA by RT-PCR and Philadephia chromosome (Ph) by conventional cytogenetic analysis. The results showed that the mean detectable rate of bcr/abl fusion gene before and after IFN-alpha therapy was 96.4% and 58.6% respectively, in 22 patients who were bcr/abl-positive before IFN-alpha therapy by D-FISH method, was 94.0% and 70.1% respectively, in 2 patients of Ph-negative before treatment. Major, minor and no responses were seen respectively in 4, 4 and 14 cases from 22 patients by D-FISH method. The results also showed a good correlation with the analysis of RT-PCR
ISSN:1009-2137