Gene diagnosis and prognosis of mediastinal lymph node occult micrometastasis in non-small cell lung carcinoma

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 24; no. 3; p. 247
• Main Authors: Wang, Zhou, Yin, Hongnian, Zhang, Lin, Lan, Xingang, Li, Houwen
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2002
• Subjects: Adult; Aged; Carcinoma, Non-Small-Cell Lung - diagnosis; Carcinoma, Non-Small-Cell Lung - secondary; Female; Genetic Markers - genetics; Humans; Lung Neoplasms - diagnosis; Lung Neoplasms - pathology; Lymphatic Metastasis - diagnosis; Male; Middle Aged; Mucin-1 - analysis; Mucin-1 - genetics; Prognosis; RNA, Messenger - analysis
• ISSN: 0253-3766

To investigate gene diagnosis of occult micrometastasis in the mediastinal lymph node in patients with non-small cell lung carcinoma (NSCLC) and to evaluate its prognostic significance. With mRNA expression of mucoid1 (MUC1) gene examined by RT-PCR, 168 mediastinal lymph nodes taken from 37 pN(0) (negative lymph nodes) NSCLC patients (stage Ia approximately IIb) made up the experiment group. Thrity negative lymph nodes from 14 benign lesions and 30 positive lymph nodes from 15 NSCLC patients served as control. The survival difference between MUC1 mRNA-negative and MUC1 mRNA-positive groups was compared by the chi(2) test. UC1 mRNA was not identified in the negative-control group (specificity = 100%), but it was identified in 26 of 30 positive-control samples (sensitivity = 86.7%). MUC1 mRNA was identified in 16 (9.5%) of the experiment group from 12 patients whose TNM stage was up-regulated to stage IIIa. The 3-year survival rate (58.3%) of MUC1 mRNA positive group patients with occult micrometastasis in medi