Comparative study of differentially expressed genes in mouse bone marrow and fetal liver cells

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 11; no. 5; p. 444
• Main Authors: Ren, Ai-Hui, Zhang, Yong, Zhao, Yan-Lin, Zhao, Lei, Liu, Xin-Yu, Zhao, Shi-Fu
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2003
• Subjects: Animals; Bone Marrow - metabolism; Female; Fetus - metabolism; Gene Expression Profiling; Hematopoiesis; Hepatocytes - metabolism; Male; Mice; Oligonucleotide Array Sequence Analysis
• ISSN: 1009-2137

Hematopoiesis undergoes several migrations from yolk sac to liver and spleen, and finally bone marrow until the end of life. A number of investigations have demonstrated that the hematopoietic microenvironment plays very important role in this process. However, the exact mechanisms remain unknown. In order to systematically analyze and understand the role of hematopoietic microenvironment in the regulation and control of hematopoiesis, a microarray containing 588 complementary DNAs was used to compare the gene expressions between those in murine fetal liver and bone marrow cells. The results obtained from array hybridization were analyzed and reconfirmed by using bioinformatics and RT-PCR as well as Northern blot. The results showed that 65 and 131 genes were relatively high expressed in bone marrow and fetal liver cells respectively among 588 known genes in the array-membrane. According to the survey in the PubMed, 39 out of bone-marrow-expressed genes and 71 in fetal-liver-expressed genes were closely relat