Comparative study of differentially expressed genes in mouse bone marrow and fetal liver cells

Hematopoiesis undergoes several migrations from yolk sac to liver and spleen, and finally bone marrow until the end of life. A number of investigations have demonstrated that the hematopoietic microenvironment plays very important role in this process. However, the exact mechanisms remain unknown. I...

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Bibliographic Details
Published inZhongguo shi yan xue ye xue za zhi Vol. 11; no. 5; p. 444
Main Authors Ren, Ai-Hui, Zhang, Yong, Zhao, Yan-Lin, Zhao, Lei, Liu, Xin-Yu, Zhao, Shi-Fu
Format Journal Article
LanguageChinese
Published China 01.10.2003
Subjects
Animals
Bone Marrow - metabolism
Female
Fetus - metabolism
Gene Expression Profiling
Hematopoiesis
Hepatocytes - metabolism
Male
Mice
Oligonucleotide Array Sequence Analysis
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Summary:Hematopoiesis undergoes several migrations from yolk sac to liver and spleen, and finally bone marrow until the end of life. A number of investigations have demonstrated that the hematopoietic microenvironment plays very important role in this process. However, the exact mechanisms remain unknown. In order to systematically analyze and understand the role of hematopoietic microenvironment in the regulation and control of hematopoiesis, a microarray containing 588 complementary DNAs was used to compare the gene expressions between those in murine fetal liver and bone marrow cells. The results obtained from array hybridization were analyzed and reconfirmed by using bioinformatics and RT-PCR as well as Northern blot. The results showed that 65 and 131 genes were relatively high expressed in bone marrow and fetal liver cells respectively among 588 known genes in the array-membrane. According to the survey in the PubMed, 39 out of bone-marrow-expressed genes and 71 in fetal-liver-expressed genes were closely relat
ISSN:1009-2137