Molecular chaperone GRP75 reprove cells from injury caused by glucose deprivation

• Published in: Shi yan sheng wu xue bao Vol. 36; no. 5; p. 381
• Main Authors: Gao, Cui Xiang, Zhang, Shuang Quan, Yin, Zhimin, Liu, Wen
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2003
• Subjects: Animals; Apoptosis; Cells, Cultured; Cricetinae; Cytoprotection; DNA, Complementary - genetics; Genetic Vectors; Glucose - deficiency; HSP70 Heat-Shock Proteins - biosynthesis; HSP70 Heat-Shock Proteins - genetics; Ischemia - complications; L-Lactate Dehydrogenase - secretion; Lung - cytology; Lung - metabolism; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Molecular Chaperones - biosynthesis; Molecular Chaperones - genetics; Rats; Reperfusion Injury - etiology; Reperfusion Injury - metabolism; Transfection
• ISSN: 0001-5334

Glucose-regulated proteins 75(grp75) is a member of hsp70 family. The expression of grp75 is upregulated during glucose starvation (such as ischemia). To evaluate grp75 function, CHL cells were cultured with glucose-free media for 20 h (A) and glucose-free media for 12 h + glucose-containing media for 8 h (ischemia reperfusion) (B). A constructed rat grp75 cDNA expression vector (pcDNA/grp75) was transfected into CHL cells and a cell strain that stably overexpressed grp75 was obtained. The transfected cells and untransfected cells(control group) were cultured with A or B. By MTT, LDH leakage measurement and flow cytometry analysis, growth rate of untransfected cells in B is significantly lower than that in glucose-containing media for 20 h (C) (p < 0.05) and A (p < 0.05). Growth rate of transfected cells is apparently higher than that of control group in B (p < 0.01). LDH liberation percentage of untransfected cells in B is obviously higher than that in C(p < 0.01) and it is not different from A(p > 0.05). LD