Expression of type II collagen gene and structural change in bone tissues of rats with experimental fluorosis

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 37; no. 4; p. 243
• Main Authors: Liu, Bing-ci, Xu, Zeng-lu, Miao, Qing, Xu, Yuan-yuan, Xu, Ming, Qian, Xiao-jing, You, Bao-rong, Yuan, Bo-hua, Kang, Ning
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2003
• Subjects: Animals; Bone Diseases - metabolism; Bone Diseases - pathology; Chondrocytes - metabolism; Collagen Type II - biosynthesis; Collagen Type II - genetics; Fluoride Poisoning - genetics; Fluoride Poisoning - metabolism; Fluoride Poisoning - pathology; Male; Rats; Rats, Wistar; RNA, Messenger - biosynthesis; RNA, Messenger - genetics
• ISSN: 0253-9624

To investigate the effects of excessive intake of fluoride on the expression of type II collagen gene and types and morphological change of collagen fiber in the bone tissues of rats. A rat model with fluorosis was established by adding 221 mg/L of sodium fluoride (NaF) to drinking water for the rats for 15 days, 30 days and two months, respectively. Type II collagen alpha1 (II) cDNA probe was prepared, and cDNA-mRNA in-situ hybridization was employed to detect change in expression of type II collagen mRNA in the bone tissues of rats with excessive intake of fluoride (221 mg/L NaF). Picrosirius-polarization method was used to observe types of collagen and morphology of collagen fiber in the bone tissues. Chondroblasts were found in the femur and other bone tissues of the rats after exposure to fluoride. cDNA-mRNA in-situ hybridization showed that expression of type II collagen gene could be observed in the cytoplasm of chondrocytic lacuna and chondrified bone tissues. mRNA in collagen of chondrocytes of the r