Detection of numerical aberrations of chromosomes 7 and 8 in sperms of workers exposed to benzene series by two-color fluorescence in situ hybridization

• Published in: Zhonghua yi xue yi chuan xue za zhi Vol. 21; no. 4; p. 360
• Main Authors: Zhao, Ting, Liu, Xin-xia, He, Yun, Deng, Li-xia, Zheng, Lu-kang
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2004
• Subjects: Adult; Benzene - poisoning; Chromosome Aberrations - chemically induced; Chromosomes, Human, Pair 7 - genetics; Chromosomes, Human, Pair 8 - drug effects; Chromosomes, Human, Pair 8 - genetics; Female; Humans; In Situ Hybridization, Fluorescence - methods; Male; Occupational Diseases - diagnosis; Occupational Diseases - genetics; Occupational Exposure - analysis; Spermatozoa - drug effects; Spermatozoa - metabolism
• ISSN: 1003-9406

To investigate the frequency of numerical aberrations for chromosomes 7 and 8 in the sperms of workers exposed to benzene series. Numerical aberrations in the sperms of workers were detected by two-color fluorescence in situ hybridization with biotin labeled chromosome 7 probe (D7Z1) and digoxingenin labeled chromosome 8 probe (D8Z1). The time-weight average air concentration (TWA) of benzene in the workshop was 42.29 mg/m(3), which was significantly higher than that of the national maximum allowable concentration [6 mg/m(3)]. The concentration of urinary trans,trans-muconic acid(ttMA) in the exposed group was also higher than that of the control group. In all, 155721 sperm nuclei from 15 workers in the exposed group and 123771 sperm nuclei from 12 individuals in the control group were examined. The results showed that the frequency of diploidy sperms and the frequencies of disomic sperm for chromosomes 7 and 8 in the exposed group (0.129%, 0.170%, 0.078%) were significantly higher than those of the control g