Liquid-based cytology as a tool for the performance of uCyt+ and Urovysion Multicolour-FISH in the detection of urothelial carcinoma

• Published in: Cytopathology (Oxford) Vol. 14; no. 6; pp. 338 - 342
• Main Authors: Mian, C, Lodde, M, Comploj, E, Negri, G, Egarter-Vigl, E, Lusuardi, L, Palermo, S, Marberger, M, Pycha, A
• Format: Journal Article
• Language: English
• Published: England 01.12.2003
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - urine; Carcinoma, Transitional Cell - diagnosis; Carcinoma, Transitional Cell - urine; Cytodiagnosis - instrumentation; Cytodiagnosis - methods; Humans; Immunohistochemistry - methods; Immunohistochemistry - standards; In Situ Hybridization, Fluorescence; Middle Aged; Predictive Value of Tests; Prospective Studies; Sensitivity and Specificity; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - urine; Urothelium - pathology
• ISSN: 0956-5507
• DOI: 10.1046/j.0956-5507.2003.00094.x

The aim of the study was to assess the value of liquid-based urinary cytology as a tool to perform uCyt+ and Multicolour-FISH in patients under follow-up after urothelial cancer. Therefore, standard cytology was compared to liquid-based cytology with the addition of the uCyt+ test, which traces the three monoclonal antibodies M344, LDQ10 and 19A211 in exfoliated urothelial cells; and Multicolour-FISH (including centromere-specific probes for chromosomes 3, 7, 17 and a locus-specific probe for 9p21/p16) performed on thin-layer specimens. UCyt+ showed an overall sensitivity of 86.2% and cytology of 45.0%. Overall sensitivity of both the tests combined was 90%. Sensitivity of Multicolour-FISH was 96.4%. All conventional cytology diagnoses were confirmed by liquid-based cytology. Liquid-based cytology is a valid tool for the performance of adjunctive analyses, such as uCyt+ and Multicolour-FISH, on residual cellular material.