Alkaline phosphatase activity in plasma of pregnant chagasic patients

The kinetic properties of plasma placental alkaline phosphatase patients with Chagas' disease were studied. When Cl2Mg was used as activator the same increase of activity (17-20%) was found in the chagasic and non chagasic groups. The enzyme was not inhibited by F-ion in any of the groups. No s...

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Published inRevista de la Facultad de Ciencias Médicas de Córdoba Vol. 55; no. 1-2; p. 5
Main Authors Sartori, M J, Lin, S, Fretes, R E, Ruiz Moreno, L, Goldemberg, L, de Fabro, S P
Format Journal Article
LanguageEnglish
Published Argentina 1997
Subjects
Adult
Alkaline Phosphatase - blood
Alkaline Phosphatase - drug effects
Alkaline Phosphatase - metabolism
Chagas Disease - blood
Chagas Disease - enzymology
Enzyme Inhibitors - pharmacology
Enzyme Reactivators - pharmacology
Female
Humans
Placenta - enzymology
Pregnancy
Pregnancy Complications, Parasitic - blood
Pregnancy Complications, Parasitic - enzymology
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Summary:The kinetic properties of plasma placental alkaline phosphatase patients with Chagas' disease were studied. When Cl2Mg was used as activator the same increase of activity (17-20%) was found in the chagasic and non chagasic groups. The enzyme was not inhibited by F-ion in any of the groups. No significant differences were detected between the two groups (chagasic and non chagasic) when the enzyme was treated with inhibitors such as EDTA and L-phenylalanine. However, when the CN-ion was used, the enzyme of the normal pregnant women followed a Michaelian curve, whereas in the chagasic group a sigmoideal plot was observed. Thus, the Hill coefficient was 1.1 for the normal group and over 1.5 for the chagasic.
ISSN:0014-6722