Immunophenotyping of 106 adult patients with acute leukemia by flow cytometry

• Published in: Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Vol. 23; no. 10; p. 1043
• Main Authors: Xu, Bing, Hu, Cheng, Miao, Xu-dong, Wu, Yan-hong, Yi, Zheng-shan, Yang, Yi, Meng, Fan-yi, Feng, Ru, Liu, Qi-fa, Liu, Xiao-li, Zheng, Wei-yang
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2003
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; CD56 Antigen - analysis; Female; Flow Cytometry - methods; Humans; Immunophenotyping; Leukemia, Myeloid, Acute - drug therapy; Leukemia, Myeloid, Acute - immunology; Leukocyte Common Antigens - analysis; Male; Middle Aged; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy; Precursor Cell Lymphoblastic Leukemia-Lymphoma - immunology; Prognosis
• ISSN: 1000-2588

To study the immunophenotyping of adult patients with acute leukemia and its association with the prognosis. Immunophenotyping was performed in 106 adult patients with acute leukemia by three-color flow cytometry analysis using CD34/SSC gating. The antigens expressed in 71 patients with acute myeloid leukemia (AML) were mainly CD13, CD33, HLA-DR, CD34 and CD117. Lymphoid antigen expression was identified in 23.9% adult AML patients and CD56 antigen expression in 15.5% of the AML patients. In 29 patients with acute lymphoblastic leukemia (ALL), the expressed antigens were mainly HLA-DR, CD10, CD19, CD34 and CD7, and 34.5% of these patients were found to be positive for myeloid antigen expression. Complete remission (CR) rate in AML patients with lymphoid antigen expression after chemotherapy was lower than that in AML patients without lymphoid antigen expression (52.9% vs 77.8%, P<0.05), and no significant impact was noted of myeloid antigen expression in ALL on the CR of the patients (70.0% vs 94.7%, P>0.05).