Methylation and expression analysis of p16(INK4a) and RB genes in meningiomas

To investigate the methylation of p16(INK4a) and RB gene, and the expression of p16(INK4a) in meningiomas. Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation of p16(INK4a) and RB in 50 cases of meningiomas, and immunostaining was performed to analyze the protein...

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Published inZhonghua yi xue yi chuan xue za zhi Vol. 21; no. 3; p. 277
Main Authors Chen, Mi-na, Mao, Qing, Liu, Yan-hui, Mao, Bo-yong
Format Journal Article
LanguageChinese
Published China 01.06.2004
Subjects
Adolescent
Adult
Aged
Aged, 80 and over
Cyclin D1 - genetics
Cyclin-Dependent Kinase 4
Cyclin-Dependent Kinases - genetics
DNA Methylation
Female
Genes, p16
Genes, Retinoblastoma
Humans
Male
Meningeal Neoplasms - genetics
Meningioma - genetics
Middle Aged
Proto-Oncogene Proteins
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Summary:To investigate the methylation of p16(INK4a) and RB gene, and the expression of p16(INK4a) in meningiomas. Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation of p16(INK4a) and RB in 50 cases of meningiomas, and immunostaining was performed to analyze the protein expression of p16(INK4a) in 25 of those cases. No methylation was found in the benign meningiomas, whereas methylation of p16(INK4a)or RB occurred in 6(37.5%) cases of grade II tumors and 4(28.6%) cases of grade III tumors, and among these cases, an atypical meningioma showed methylation of both genes. Thirteen cases showed p16(INK4a) positive expression, but none of them was methylated. The methylation of p16(INK4a) or RB is related with the tumorigenesis and progression of atypical and anaplastic meningiomas, and a probable mechanism is that methylation causes the loss of expression and leads to dysfuncation of the p16(INK4a)/cyclin D1/CDK4/RB pathway.
ISSN:1003-9406