Influence of the watery extract of jiangzhining decoction on the genetic expression of hepatocyte LDLR of hyperlipidemic rats

• Published in: Zhongguo zhongyao zazhi Vol. 27; no. 4; p. 289
• Main Authors: Guan, Jian-hong, Liang, Ai-hua, Feng, Qian-jin, Hu, Shan-shan
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2002
• Subjects: Actins - biosynthesis; Actins - genetics; Animals; Drug Combinations; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; Hyperglycemia - metabolism; Hypoglycemic Agents - administration & dosage; Hypoglycemic Agents - pharmacology; Liver - metabolism; Male; Rats; Rats, Wistar; Receptors, LDL - biosynthesis; Receptors, LDL - genetics; RNA, Messenger - genetics
• ISSN: 1001-5302

To study the influence of Jiangzhining decoction on the genetic expression of Liver LDLR of the rats suffered from hyperlipemia. Laboratory animals were male wister rats with hyperlipemia resulting from high fat feeding. Prescription was the douche of stomach with Jiangzhining decoction (200%) with a dosage of 1.4 g.kg-1, for 15 successive days. Total RNA was extracted from the liver tissue of treated rats and LDLRmRNA was detected by Dot blot hybridization. Expression levels of LDLRmRNA was estimated by a ratio of LDLRmRNA and beta-actin mRNA. The difference between expression levels of LDLRmRNA for normal group and those for hyperlipemia group (100% +/- 19% vs 39% +/- 14%) was significant (P < 0.05); and the difference between decoction group (108 +/- 8%) and hyperlipimia group was also highly significant (P < 0.01). High fat feeding reduces the expression of liver LDLRmRNA while the decoction can greatly increase it. The study and development of Jiangzhining are significant in preventing and curing cadioce