C5b-9 deposition in children with glomerular diseases

Kidney biopsies were obtained in 28 children with glomerular diseases and studied using indirect immunofluoreoscence and immunoperoxidase for the detection of IgG, IgA, IgM, Clq, C3c, C4, Fibrinogen and the neoantigens of the terminal C5b-9 complement complex. An affinity-purified rabbit IgG was use...

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Bibliographic Details
Published inMedecine interne Vol. 25; no. 2; p. 99
Main Authors Nanulescu, M, Rus, H G, Niculescu, F, Cristea, A, Florescu, P
Format Journal Article
LanguageEnglish
Published Romania 01.04.1987
Subjects
Adolescent
Biopsy
Child
Child, Preschool
Complement Membrane Attack Complex
Complement System Proteins - immunology
Female
Fluorescent Antibody Technique
Glomerulonephritis - immunology
Glomerulosclerosis, Focal Segmental - immunology
Humans
Immunoenzyme Techniques
Kidney Glomerulus - pathology
Male
Nephrosis, Lipoid - immunology
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Summary:Kidney biopsies were obtained in 28 children with glomerular diseases and studied using indirect immunofluoreoscence and immunoperoxidase for the detection of IgG, IgA, IgM, Clq, C3c, C4, Fibrinogen and the neoantigens of the terminal C5b-9 complement complex. An affinity-purified rabbit IgG was used to recognize the neoantigens of the assembled terminal components of the complement system into the C5b-9 complex. The immunohistochemical studies were correlated to the clinical data and laboratory investigations. Fourteen of the 28 patients presented specific C5b-9 glomerular deposits; they were also present at the tubular sites in 8 patients and at the vascular sites in 12 patients. An unfavourable evolution was observed for patients presenting C5b-9 deposits in contrast to those without such deposits, even of the same histopathological pattern of glomerulonephritis. The presence of C5b-9 complex at the site of glomerular injury suggests a pathological involvement of the "in situ" complement activation and could be a marker in the prognosis of the glomerular diseases.
ISSN:0377-1202