Effect of prolactin on phosphatidylcholine hydrolysis via phospholipase C in isolated adrenocortical cells of guinea pig

Prolactin significantly increased the rate of incorporation of [3H]choline into phosphorylcholine (PRC) in a purified suspension of guinea pig adrenocortical cells. The rate of phosphatidylcholine (PTC) labelling and cellular PTC content did not change. In cells prelabelled with [3H]choline or CDP [...

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Bibliographic Details
Published inBiomedical science Vol. 1; no. 2; p. 178
Main Authors Sautin YuY, Tronko, N D, Mikosha, A S
Format Journal Article
LanguageEnglish
Published England 01.02.1990
Subjects
Adrenal Cortex - cytology
Adrenal Cortex - metabolism
Animals
Cells, Cultured
Diglycerides - metabolism
Guinea Pigs
Hydrolysis
Kinetics
Phosphatidylcholines - metabolism
Phosphorylcholine - metabolism
Prolactin - physiology
Type C Phospholipases - metabolism
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Summary:Prolactin significantly increased the rate of incorporation of [3H]choline into phosphorylcholine (PRC) in a purified suspension of guinea pig adrenocortical cells. The rate of phosphatidylcholine (PTC) labelling and cellular PTC content did not change. In cells prelabelled with [3H]choline or CDP [14C]choline, prolactin diminished the rate of reduction of the radioactive PRC pool after 60-90 min incubation without any change in the rate of PTC biosynthesis. Taken together, these findings suggest that prolactin stimulates the hydrolysis of PTC by phospholipase C into PRC and diacylglycerol. The significance of this effect as part of the mechanism of action of prolactin on adrenocortical cells is discussed.
ISSN:0955-9701