Platelet-aggregating activities of metastasizing tumor cells. III. Platelet aggregation as resulting from thrombin generation by tumor cells

Thrombin generated in the process of platelet aggregation induced by three metastasizing murine tumors was measured using a chromogenic substrate specific for thrombin. Addition of B16 cells or 3LL cells to the platelet-rich plasma induced the generation of a significant amount of thrombin during th...

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Bibliographic Details
Published inInvasion and metastasis Vol. 5; no. 2; p. 96
Main Authors Tohgo, A, Tanaka, N G, Ogawa, H
Format Journal Article
LanguageEnglish
Published Switzerland 1985
Subjects
Animals
Blood Coagulation Factors - physiology
Calcification, Physiologic
Cell Line
Chromogenic Compounds
Dipeptides
Male
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Neoplasm Metastasis - blood
Platelet Aggregation
Thrombin - biosynthesis
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Summary:Thrombin generated in the process of platelet aggregation induced by three metastasizing murine tumors was measured using a chromogenic substrate specific for thrombin. Addition of B16 cells or 3LL cells to the platelet-rich plasma induced the generation of a significant amount of thrombin during the lag period preceding aggregation, while that of MH134 cells did not. Thrombin generation was observed in both the presence and the absence of platelets, indicating that platelets are not necessarily required for thrombin generation by these tumor cells. This suggests that the adherence of platelets to tumor cells is not an essential step for the initiation of thrombin-mediated aggregation induced by B16 and 3LL cells. Furthermore, the results of a one-stage clotting assay using plasma deficient in coagulation factors indicated that B16 and 3LL cells generated thrombin through direct activation of factor X.
ISSN:0251-1789