Synaptogenesis and axon collaterals coming from the white matter in the upper cervical cord of the 10 day (stage 36) chick embryo--Golgi and electron microscopic studies

• Published in: No To Shinkei Vol. 39; no. 9; p. 869
• Main Authors: Matsuda, S, Nakasone, T, Kanemitsu, A
• Format: Journal Article
• Language: Japanese
• Published: Japan 01.09.1987
• Subjects: Animals; Axons - anatomy & histology; Axons - embryology; Chick Embryo; Spinal Cord - anatomy & histology; Spinal Cord - embryology; Synapses - anatomy & histology; Synapses - embryology
• ISSN: 0006-8969

Our previous study with 3H-thymidine autoradiography showed that neurons of the zona spongiosa, the nucleus proprius of the dorsal horn, the zona intermedia and the ventralhorn differentiated earlier than those of the substantia gelatinosa and the neck and the base of the dorsal horn, and that neurons of the substantia gelatinosa which were the last to differentiate reached their final position at stage 36 (Fig. 1). In the upper cervical cord of chick embryos at stage 36 when all spinal neurons finished cell migration and the cytoarchitecture similar to that of the cat spinal cord (Rexed, 1952) could be recognized (cf. Figs. 1, 3B), we studied the distribution of synapses by the electron microphotomontage (Fig. 3 A) and the morphology of axon collaterals coming from the white matter by the Golgi method (Fig. 4), in order to examine i) which spinal neurons have synaptic contacts at this stage and ii) what part of the axon collateral makes synaptic contacts. In the white matter, synapses were numerous around the gray matter and they were few in the peripheral part along the external surface of the cord. The paucity of synapses in the peripheral part was explained by a finding that dendrites reaching the external surface of the cord were few in number at this stage (cf. Fig. 3 C). In the gray matter, synapses were more numerous and denser in the zona intermedia and the ventral horn than in the dorsal horn.(ABSTRACT TRUNCATED AT 250 WORDS)