Purification and characterization of phytomitogens isolated from Phaseolus vulgaris bean

The physical properties and biological activities of commercial PHAP (Difco) with eleven phytoproteins purified from red kidney beans (Phaseolus vulgaris) are compared. In gel filtration pattern, there seems no apparent difference among F2, F9 and F10. However, F1 component appears second sharp peak...

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Bibliographic Details
Published inZhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Vol. 15; no. 3; p. 233
Main Author Chiang, L C
Format Journal Article
LanguageChinese
Published China (Republic : 1949- ) 01.08.1982
Subjects
Cell Division
Chromatography, Affinity
Chromatography, Gel
Electrophoresis
Fabaceae - analysis
Humans
Lymphocyte Activation
Phytohemagglutinins - isolation & purification
Plant Lectins
Plants, Medicinal
Urinary Bladder Neoplasms - immunology
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Summary:The physical properties and biological activities of commercial PHAP (Difco) with eleven phytoproteins purified from red kidney beans (Phaseolus vulgaris) are compared. In gel filtration pattern, there seems no apparent difference among F2, F9 and F10. However, F1 component appears second sharp peak which given no biological activity. From results of cellulose acetate electrophoresis, it showed that majority of F1, F5, and F7 are anodal proteins, while only minor part of F2, F9, F10 and F12 possess this property. As to biological activities, higher potency for hemagglutination are obtained from F8(467%), F12(350%0, F9(280%) and F10(100%). On the other hand, fractions such as F10(90%), F2(87%), F8(86%) and F12(64%) reveal more capability of causing lymphocyte mitogenesis. For lymphocyte transformation, F10 lymphocyte transformation, F10(100%), F9(96%), F2(93%), F3(86%), F5(84%), F1(80%), F8(78%), and F12(70%) are predominance in their activity. In lymphocyte transformation test, F9 and F10 exhibit highest acti
ISSN:0253-2662